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rabbit polyclonal antibody against col ii (Danaher Inc)

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Danaher Inc rabbit polyclonal antibody against col ii
Matrix deposition after 28 days of subcutaneous implantation. Representative immunohistochemical staining of the hydrogel samples for <t>COL</t> II. Positive protein expression stained in dark brown. The left panel in each condition shows 10× magnification pictures (scale bars represent 250 µm), whereas the right panel shows 20× (scale bars represent 100 µm).

Matrix deposition after 28 days of subcutaneous implantation. Representative immunohistochemical staining of the hydrogel samples for <t>COL</t> II. Positive protein expression stained in dark brown. The left panel in each condition shows 10× magnification pictures (scale bars represent 250 µm), whereas the right panel shows 20× (scale bars represent 100 µm).

Rabbit Polyclonal Antibody Against Col Ii, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 20243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibody+against+col+ii/Rabbit+Polyclonal+Anti-JAK2+(phospho+Y1007)+antibody/pmc09607606-89-10-16
Average 99 stars, based on 20243 article reviews

rabbit polyclonal antibody against col ii - by Bioz Stars, 2026-09

99/100 stars

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1) Product Images from "Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration"

Article Title: Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration

Journal: Polymers

doi: 10.3390/polym14204292

Matrix deposition after 28 days of subcutaneous implantation. Representative immunohistochemical staining of the hydrogel samples for COL II. Positive protein expression stained in dark brown. The left panel in each condition shows 10× magnification pictures (scale bars represent 250 µm), whereas the right panel shows 20× (scale bars represent 100 µm).

Figure Legend Snippet: Matrix deposition after 28 days of subcutaneous implantation. Representative immunohistochemical staining of the hydrogel samples for COL II. Positive protein expression stained in dark brown. The left panel in each condition shows 10× magnification pictures (scale bars represent 250 µm), whereas the right panel shows 20× (scale bars represent 100 µm).

Techniques Used: Immunohistochemical staining, Staining, Expressing

Incubation:

Article Title: Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration
Article Snippet: .. Slides were subsequently incubated overnight at 4 °C with a rabbit polyclonal antibody against COL II (Abcam, Cambridge, UK). .. Thereafter, sections were incubated with polyclonal goat anti-rabbit HRP-conjugated secondary antibody (Dako) for 30 min at room temperature, followed by development with the DAB Substrate Kit (Abcam).

Article Title: Dominant role of in situ native cartilage niche for determining the cartilage type regenerated by BMSCs
Article Snippet: For histological analysis, 4,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, USA) and hematoxylin and eosin (HE) staining were performed to observe the nucleus, and Safranin O (SO) was used to detect the deposition of GAG. .. For immunohistochemical analysis, a rabbit polyclonal antibody against Col II (1:100 in PBS; ab34712, Abcam, Cambridge, MA, USA) was used to detect the expression of Col II, followed by incubated with an HRP-conjugated anti-rabbit secondary antibody (1:100; Dako, Glostrup, Denmark). .. Diaminobenzidine tetrahydrochloride (DAB, Santa Cruz Biotechnology) was used to develop color, thereby aiding visualization, according to a previously established method [ ].

Article Title: Engineering of Optimized Hydrogel Formulations for Cartilage Repair
Article Snippet: .. Slides were subsequently incubated overnight at 4 °C with a rabbit polyclonal antibody against COL II (Abcam, Cambridge, UK). .. The sections were then incubated with a polyclonal goat–anti-rabbit HRP-conjugated secondary antibody (Dako, Glostrup, Denmark), followed by development with the DAB Substrate kit (Abcam).

Staining:

Article Title: EZH2 is associated with cartilage degeneration in osteoarthritis by promoting SDC1 expression via histone methylation of the microRNA-138 promoter.
Article Snippet: Cartilage degeneration has been reported to deteriorate osteoarthritis (OA), a prevalent joint disease caused by intrinsic and epigenetic factors.. This study aimed to examine the molecular mechanism of enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2)/microRNA-138 (miR-138)/syndecan 1 (SDC1) and its epigenetic regulation in cartilage degeneration in OA.. An OA cell model was induced by stimulating chondrocytes with interleukin (IL)-1β at a final concentration of 10 ng/mL, followed by alterations in EZH2 and miR-138 expression.

Immunohistochemical staining:

Expressing:

Article Title: Cartilage Regeneration Characteristics of Human and Goat Auricular Chondrocytes
Article Snippet: .. A rabbit polyclonal antibody against Col II (1:100 in PBS; ab34712, Abcam, Cambridge, MA, United States), followed by an HRP-conjugated anti-rabbit secondary antibody (1:100; Dako, Glostrup, Denmark), both diluted in PBS, and then colorized with diaminobenzidine tetrahydrochloride (DAB, Santa Cruz Biotechnology) were used to detect the expression of Col II ( ). .. Immunohistochemical analyses of COL I and COL X was performed using rabbit polyclonal antibody (1:500 in PBS; GB11022, Servicebio, China), and rabbit polyclonal antibody (1:200 in PBS; DF13214, Affinity Biosciences, United States).

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Journal: Polymers

Article Title: Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration

doi: 10.3390/polym14204292

Figure Lengend Snippet: Matrix deposition after 28 days of subcutaneous implantation. Representative immunohistochemical staining of the hydrogel samples for COL II. Positive protein expression stained in dark brown. The left panel in each condition shows 10× magnification pictures (scale bars represent 250 µm), whereas the right panel shows 20× (scale bars represent 100 µm).

Article Snippet: Slides were subsequently incubated overnight at 4 °C with a rabbit polyclonal antibody against COL II (Abcam, Cambridge, UK).

Techniques: Immunohistochemical staining, Staining, Expressing

The expression of HO-1 in human nucleus pulposus tissues ( a ) Disc tissues from patients were collected and classified according to the modified Pfirrmann grading system, and samples of grade II/III (n = 20) were assigned to the mild IDD group, while samples of grade IV/V (n = 20) were allocated to the severe IDD group. ( b ) Real-time PCR of human degenerated intervertebral disc samples showed that the expression of HO-1 is consistently higher in the Grade II and Grade III (mild degeneration) samples than in Grade IV and Grade V (severe degeneration) samples. The results were normalized to the expression of GAPDH. (n = 40, mild IDD = 20, severe IDD = 20). ( c ) We further pooled the samples into two groups according to the clinical significance (mild and severe IDD), and the whisker box plot showed the HO-1 expression is considerably higher in mild than severe groups. ( d ) Gene expression of HO-1 was negatively correlated with the Pfirrmann grades of 40 human NP tissues (n = 40, r = −0.47). Immunohistochemistry of the human NP sections showed that numbers of HO-1 ( e ) and COL-II ( f ) -positive cells in the mild IDD group were remarkably decreased compared with those in the severe IDD group. The number of cells immunopositive for MMP-1 ( g ) were substantially higher in severe IDD compared with those in mild IDD NP tissues. Values represent the mean and standard deviation (SD). * P < 0.05, bars = 100 μm.

Journal: Scientific Reports

Article Title: Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration

doi: 10.1038/srep21190

Figure Lengend Snippet: The expression of HO-1 in human nucleus pulposus tissues ( a ) Disc tissues from patients were collected and classified according to the modified Pfirrmann grading system, and samples of grade II/III (n = 20) were assigned to the mild IDD group, while samples of grade IV/V (n = 20) were allocated to the severe IDD group. ( b ) Real-time PCR of human degenerated intervertebral disc samples showed that the expression of HO-1 is consistently higher in the Grade II and Grade III (mild degeneration) samples than in Grade IV and Grade V (severe degeneration) samples. The results were normalized to the expression of GAPDH. (n = 40, mild IDD = 20, severe IDD = 20). ( c ) We further pooled the samples into two groups according to the clinical significance (mild and severe IDD), and the whisker box plot showed the HO-1 expression is considerably higher in mild than severe groups. ( d ) Gene expression of HO-1 was negatively correlated with the Pfirrmann grades of 40 human NP tissues (n = 40, r = −0.47). Immunohistochemistry of the human NP sections showed that numbers of HO-1 ( e ) and COL-II ( f ) -positive cells in the mild IDD group were remarkably decreased compared with those in the severe IDD group. The number of cells immunopositive for MMP-1 ( g ) were substantially higher in severe IDD compared with those in mild IDD NP tissues. Values represent the mean and standard deviation (SD). * P < 0.05, bars = 100 μm.

Article Snippet: Next, the sections were incubated at 4 °C overnight with the following primary antibodies: rabbit polyclonal antibody against HO-1 (1:100 dilution), rabbit polyclonal antibody against COL-II (1:100 dilution), and rabbit polyclonal antibody against MMP-1 (1:100 dilution) (Proteintech, Chicago, IL, USA).

Techniques: Expressing, Modification, Real-time Polymerase Chain Reaction, Whisker Assay, Gene Expression, Immunohistochemistry, Standard Deviation

CoPP rescues ECM anabolic gene expression and collagen type II deposition in IL-1β-treated cells ( a ) Immunocytochemistry assay revealed that IL-1β treatment downregulated the expression of COL-II in human NP cells (a2). Stimulation with the HO-1 inducer CoPP partially abolished the effect of IL-1β and prevented the loss of COL-II deposition induced by IL-1β (a4,a6). Bars = 100 μm (a1–a5) or 50 μm (a6). Real-time PCR showed that IL-1β treatment led to a remarkable downregulation of gene expression of aggrecan ( b ), COL-II ( c ) and SOX-6 ( d ) in human NP cells, and addition of CoPP to the cells treated with IL-1β notably increased the gene expression of aggrecan, COL-II and SOX-6 although they did not reach basal levels. ( e ) Treatment with IL-1β did not affect the gene expression of SOX-9 in human NP cells. Values represent the mean and SD. * P < 0.05 with respect to IL-1β.

Journal: Scientific Reports

Article Title: Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration

doi: 10.1038/srep21190

Figure Lengend Snippet: CoPP rescues ECM anabolic gene expression and collagen type II deposition in IL-1β-treated cells ( a ) Immunocytochemistry assay revealed that IL-1β treatment downregulated the expression of COL-II in human NP cells (a2). Stimulation with the HO-1 inducer CoPP partially abolished the effect of IL-1β and prevented the loss of COL-II deposition induced by IL-1β (a4,a6). Bars = 100 μm (a1–a5) or 50 μm (a6). Real-time PCR showed that IL-1β treatment led to a remarkable downregulation of gene expression of aggrecan ( b ), COL-II ( c ) and SOX-6 ( d ) in human NP cells, and addition of CoPP to the cells treated with IL-1β notably increased the gene expression of aggrecan, COL-II and SOX-6 although they did not reach basal levels. ( e ) Treatment with IL-1β did not affect the gene expression of SOX-9 in human NP cells. Values represent the mean and SD. * P < 0.05 with respect to IL-1β.

Techniques: Gene Expression, Immunocytochemistry, Expressing, Real-time Polymerase Chain Reaction

CoPP attenuates the effects of IL-1β on anabolic and catabolic activities mainly depend on HO-1 induction ( a ) Western blot and real-time PCR analysis revealed that HO-1 siRNA treatment inhibitied HO-1 expression compared with a scramble siRNA that targets none of the related genes as negative control. ( b ) si-HO-1 treatment attenuated the effect of CoPP on the inhibition of CS synthesis induced by IL-1β (bars = 100 μm). ( c ) HO-1 siRNA treatment substantially weakened the effect of CoPP on the down regulation of anabolic genes (Aggrecan, COL-II and SOX-6) induced by IL-1β. ( d,e ) HO-1 siRNA treatment considerably subsided the effect of CoPP on the increment of catabolic genes (MMP-1, MMP-3, MMP-9, MMP-13, ADAMTS-5 and ADAMTS-4) and proteins (MMP-1, MMP-3, MMP-9 and MMP-13) induced by IL-1β. Values represent the mean and SD. * P < 0.05 with respect to IL-1β+ CoPP+ siNC.

Journal: Scientific Reports

Article Title: Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration

doi: 10.1038/srep21190

Figure Lengend Snippet: CoPP attenuates the effects of IL-1β on anabolic and catabolic activities mainly depend on HO-1 induction ( a ) Western blot and real-time PCR analysis revealed that HO-1 siRNA treatment inhibitied HO-1 expression compared with a scramble siRNA that targets none of the related genes as negative control. ( b ) si-HO-1 treatment attenuated the effect of CoPP on the inhibition of CS synthesis induced by IL-1β (bars = 100 μm). ( c ) HO-1 siRNA treatment substantially weakened the effect of CoPP on the down regulation of anabolic genes (Aggrecan, COL-II and SOX-6) induced by IL-1β. ( d,e ) HO-1 siRNA treatment considerably subsided the effect of CoPP on the increment of catabolic genes (MMP-1, MMP-3, MMP-9, MMP-13, ADAMTS-5 and ADAMTS-4) and proteins (MMP-1, MMP-3, MMP-9 and MMP-13) induced by IL-1β. Values represent the mean and SD. * P < 0.05 with respect to IL-1β+ CoPP+ siNC.

Techniques: Western Blot, Real-time Polymerase Chain Reaction, Expressing, Negative Control, Inhibition

NOX/ROS regulation on the effect of HO-1 induction ( a ) Western blot analysis revealed that the increased expression of NOX4 induced by IL-1β was ameliorated by DPI or NAC, but not CoPP treatment; the IL-1β dependent activation of MEK1/2 and ERK1/2 was remarkably lowered by DPI, NAC or CoPP treatment; However, HO-1 siRNA did not alter the effect of DPI or NAC on NOX4 protein expression. ( b ) Induction of HO-1 decreased, while HO-1 knockdown increased, the elevated ROS level which stimulated by IL-1β. ( c ) Treatment of DPI or NAC substantially attenuated the increased ROS level which induced by IL-1β. ( d,e ) DPI or NAC treatment considerably weakened the effect of IL-1β on the down regulation of anabolic genes (aggrecan and COL-II) and up regulation of catabolic genes (MMP-1 and MMP-13) expression. ( f ) A summary of the mechanism of COPP in regulating MEK/ERK signaling and its downstream ECM catabolic effectors through ROS neutralizing. Values represent the mean and SD. * P < 0.05 with respect to IL-1β + DMSO (0.1%).

Journal: Scientific Reports

Article Title: Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration

doi: 10.1038/srep21190

Figure Lengend Snippet: NOX/ROS regulation on the effect of HO-1 induction ( a ) Western blot analysis revealed that the increased expression of NOX4 induced by IL-1β was ameliorated by DPI or NAC, but not CoPP treatment; the IL-1β dependent activation of MEK1/2 and ERK1/2 was remarkably lowered by DPI, NAC or CoPP treatment; However, HO-1 siRNA did not alter the effect of DPI or NAC on NOX4 protein expression. ( b ) Induction of HO-1 decreased, while HO-1 knockdown increased, the elevated ROS level which stimulated by IL-1β. ( c ) Treatment of DPI or NAC substantially attenuated the increased ROS level which induced by IL-1β. ( d,e ) DPI or NAC treatment considerably weakened the effect of IL-1β on the down regulation of anabolic genes (aggrecan and COL-II) and up regulation of catabolic genes (MMP-1 and MMP-13) expression. ( f ) A summary of the mechanism of COPP in regulating MEK/ERK signaling and its downstream ECM catabolic effectors through ROS neutralizing. Values represent the mean and SD. * P < 0.05 with respect to IL-1β + DMSO (0.1%).

Techniques: Western Blot, Expressing, Activation Assay, Knockdown

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rabbit polyclonal antibody against col ii (Danaher Inc)

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1) Product Images from "Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration"

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